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College of Human Sciences

Optimizing Human Performance

Clinical Skeletal Muscle Physiology and Biochemistry Laboratory

About the lab

The Clinical Skeletal Muscle Physiology and Biochemistry Lab is a research facility that is part of the Nutrition, Food & Exercise Sciences Department of the College of Human Sciences at Florida State University in Tallahassee, Florida.

Our lab studies different aspects of muscle physiology and biochemistry in diseased individuals, with a focus on the development of strategies to improve skeletal muscle tissue in the legs of patients suffering from peripheral artery disease (PAD). We evaluate the mechanisms that produce the leg dysfunction of PAD utilizing experimental and computational modeling including cell culture, animal studies and human clinical studies.

Research Interests

Cytoskeletal proteins 

Evaluation and association with muscle morphology

Oxidative stress

Signaling mechanisms in protein modification under ischemia-reperfusion conditions.

Antioxidant defenses

Mitochondrial function ​

Electron transport chain complex activity and ATP production

Calcium protease system

Effects of revascularization operations and supervised exercise treatment

lab diagram

Our research focuses on the development of improved diagnostic and treatment methods for patients with peripheral artery disease (PAD). PAD affects 8 million people in the USA and is a manifestation of atherosclerosis that produces progressive narrowing and occlusion of the arteries supplying the lower extremities. The most common clinical manifestation of PAD is claudication, i.e., a severe functional limitation identified as gait dysfunction and walking-induced leg muscle pain. A myopathy produced by chronic ischemia is present in the legs of patients with PAD and is central to the functional deterioration seen in claudication.

Faculty / Staff

Panagiotis Koutakis, Ph.D.

Assistant Professor, Lab Director
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Eva Papoutsi

Research Assistant


Ahmed Ismaeel

Ph.D. Student, Exercise Physiology

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Jeremiah Kidd

M.S. Student, Exercise Physiology

Luke Galloway

Undergraduate Student

Katherine Jones

Undergraduate Student

Katie Prezioso

Undergraduate Student


Dr. Ramon Lavado, PhD

Baylor University
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Dr. Jeffrey S. Kirk, MD

Capital Regional Medical Center
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Dr. W. Todd Bohannon, MD

Baylor Scott and White Health
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Dr. Iraklis I. Pipinos, MD, PhD

University of Nebraska at Medical Center
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wdt_ID Title Author Journal Link
1 Effects of limb revascularization procedures on oxidative stress Ismaeel A, Lavado R, Smith RS, Eidson JL, Sawicki I., Kirk JS., Bohannon WT, Koutakis P. J Surg Res. 2018 Dec; 232: 503-509 Review
2 Quantification of Daily Physical Activity and Sedentary Behavior of Claudicating Patients Hernandez H, Myers SA, Schieber M, Ha DM, Baker S, Koutakis P, Kim KS, Mietus C, Casale GP, Pipinos II Ann Vasc Surg. 2018 Aug 13. pii: S0890-5096(18)30598-3
3 Oxidative stress and antioxidant treatment in patients with peripheral artery disease Koutakis P, Ismaeel A, Farmer P, Purcell S, Smith RS, Eidson JL, Bohannon WT Physiol Rep. 2018 Apr;6(7):e13650
4 Chloroquine improves the response to ischemic muscle injury and increases HMGB1 after arterial ligation Xu J, Cui X, Li J, Koutakis P, Pipinos I, Tzeng E, Chen A, Sachdev U Vasc Surg. 2018 Mar;67(3):910-921
5 Premature aortic smooth muscle cell differentiation contributes to matrix dysregulation in Marfan Syndrome Dale M, Fitzgerald MP, Liu Z, Meisinger T, Karpisek A, Purcell LN, Carson JS, Harding P, Lang H, Koutakis P, Batra R, Mietus CJ, Casale G, Pipinos I, Baxter BT, Xiong W PLoS One. 2017 Oct 17;12(10):e0186603
6 An endovascular model of ischemic myopathy from peripheral arterial disease Long CA, Timmins LH, Koutakis P, Goodchild TT, Lefer DJ, Pipinos II, Casale GP, Brewster LP J Transl Med. 2016 Feb 4;14(1):39
7 Patient Demographics And Cardiovascular Risk Factors Differentially Influence Geometric Remodeling Of The Aorta Compared To The Peripheral Arteries Kamenskiy, A., Miserlis, D., Adamson, P., Adamson, M., Knowles, T., Neme, J., Koutakis, P., Philips, N., Pipinos, I., MacTaggart, J Surgery. 2015 Jun 19. pii: S0039-6060(15)00425-0
8 Abnormal Myofiber Morphology and Limb Dysfunction in Claudication Koutakis, P., Myers, S.A, Cluff, K., Ha D.M., Haynatzki, G., McComb R.D., Uchida, K., Miserlis, D., Papoutsi, E., Johanning, J.M., Casale, G.P. Pipinos, I.I J Surg Res. 2015 Jun 1;196(1):172-9
9 Abnormal accumulation of desmin in gastrocnemius myofibers of patients with peripheral artery disease: associations with altered myofiber morphology and density, mitochondrial dysfunction and impaired limb function Koutakis, P., Casale, G.P., Kim, J.K.S., Miserlis, D., Zhu, Z., Papoutsi, E., Ha, D.M., Swanson, S.A., Carpenter, L.A., Myers, S.A., Johanning, J.M, Pipinos, I.I J Histochem Cytochem. 2015 Jan 9. pii: 0022155415569348
10 Reliability and Validity of a Wireless Accelerometer for the Assessment of Postural Sway Saunders, N.W., Koutakis, P, Kloos, A.D., Kegelmeyer, D.A., Dicke, J.D., Devor S.T J Appl Biomech. 2015 Jan 5

Get in Touch

  4014 William Johnston Bldg.
143 Honors Way
Tallahassee, FL 32301