Clinical Skeletal Muscle Physiology and Biochemistry Laboratory
About the lab
The Clinical Skeletal Muscle Physiology and Biochemistry Lab is a research facility that is part of the Nutrition, Food & Exercise Sciences Department of the College of Human Sciences at Florida State University in Tallahassee, Florida.
Our lab studies different aspects of muscle physiology and biochemistry in diseased individuals, with a focus on the development of strategies to improve skeletal muscle tissue in the legs of patients suffering from peripheral artery disease (PAD). We evaluate the mechanisms that produce the leg dysfunction of PAD utilizing experimental and computational modeling including cell culture, animal studies and human clinical studies.
Research Interests
Cytoskeletal proteins
Evaluation and association with muscle morphology
Signaling mechanisms in protein modification under ischemia-reperfusion conditions.
Antioxidant defenses
Electron transport chain complex activity and ATP production
Effects of revascularization operations and supervised exercise treatment
Our research focuses on the development of improved diagnostic and treatment methods for patients with peripheral artery disease (PAD). PAD affects 8 million people in the USA and is a manifestation of atherosclerosis that produces progressive narrowing and occlusion of the arteries supplying the lower extremities. The most common clinical manifestation of PAD is claudication, i.e., a severe functional limitation identified as gait dysfunction and walking-induced leg muscle pain. A myopathy produced by chronic ischemia is present in the legs of patients with PAD and is central to the functional deterioration seen in claudication.
Faculty / Staff
Students
Jeremiah Kidd
M.S. Student, Exercise Physiology
Luke Galloway
Undergraduate Student
Katherine Jones
Undergraduate Student
Katie Prezioso
Undergraduate Student
Collaborators
Publications
| wdt_ID | Title | Author | Journal | Link |
|---|---|---|---|---|
| 1 | Effects of limb revascularization procedures on oxidative stress | Ismaeel A, Lavado R, Smith RS, Eidson JL, Sawicki I., Kirk JS., Bohannon WT, Koutakis P. | J Surg Res. 2018 Dec; 232: 503-509 Review | https://www.sciencedirect.com/science/journal/00224804 |
| 2 | Quantification of Daily Physical Activity and Sedentary Behavior of Claudicating Patients | Hernandez H, Myers SA, Schieber M, Ha DM, Baker S, Koutakis P, Kim KS, Mietus C, Casale GP, Pipinos II | Ann Vasc Surg. 2018 Aug 13. pii: S0890-5096(18)30598-3 | https://www.ncbi.nlm.nih.gov/pubmed/30114505 |
| 3 | Oxidative stress and antioxidant treatment in patients with peripheral artery disease | Koutakis P, Ismaeel A, Farmer P, Purcell S, Smith RS, Eidson JL, Bohannon WT | Physiol Rep. 2018 Apr;6(7):e13650 | |
| 4 | Chloroquine improves the response to ischemic muscle injury and increases HMGB1 after arterial ligation | Xu J, Cui X, Li J, Koutakis P, Pipinos I, Tzeng E, Chen A, Sachdev U | Vasc Surg. 2018 Mar;67(3):910-921 | |
| 5 | Premature aortic smooth muscle cell differentiation contributes to matrix dysregulation in Marfan Syndrome | Dale M, Fitzgerald MP, Liu Z, Meisinger T, Karpisek A, Purcell LN, Carson JS, Harding P, Lang H, Koutakis P, Batra R, Mietus CJ, Casale G, Pipinos I, Baxter BT, Xiong W | PLoS One. 2017 Oct 17;12(10):e0186603 | |
| 6 | An endovascular model of ischemic myopathy from peripheral arterial disease | Long CA, Timmins LH, Koutakis P, Goodchild TT, Lefer DJ, Pipinos II, Casale GP, Brewster LP | J Transl Med. 2016 Feb 4;14(1):39 | |
| 7 | Patient Demographics And Cardiovascular Risk Factors Differentially Influence Geometric Remodeling Of The Aorta Compared To The Peripheral Arteries | Kamenskiy, A., Miserlis, D., Adamson, P., Adamson, M., Knowles, T., Neme, J., Koutakis, P., Philips, N., Pipinos, I., MacTaggart, J | Surgery. 2015 Jun 19. pii: S0039-6060(15)00425-0 | |
| 8 | Abnormal Myofiber Morphology and Limb Dysfunction in Claudication | Koutakis, P., Myers, S.A, Cluff, K., Ha D.M., Haynatzki, G., McComb R.D., Uchida, K., Miserlis, D., Papoutsi, E., Johanning, J.M., Casale, G.P. Pipinos, I.I | J Surg Res. 2015 Jun 1;196(1):172-9 | |
| 9 | Abnormal accumulation of desmin in gastrocnemius myofibers of patients with peripheral artery disease: associations with altered myofiber morphology and density, mitochondrial dysfunction and impaired limb function | Koutakis, P., Casale, G.P., Kim, J.K.S., Miserlis, D., Zhu, Z., Papoutsi, E., Ha, D.M., Swanson, S.A., Carpenter, L.A., Myers, S.A., Johanning, J.M, Pipinos, I.I | J Histochem Cytochem. 2015 Jan 9. pii: 0022155415569348 | https://www.ncbi.nlm.nih.gov/pubmed/25575565 |
| 10 | Reliability and Validity of a Wireless Accelerometer for the Assessment of Postural Sway | Saunders, N.W., Koutakis, P, Kloos, A.D., Kegelmeyer, D.A., Dicke, J.D., Devor S.T | J Appl Biomech. 2015 Jan 5 |
Get in Touch
| 4014 William Johnston Bldg. 143 Honors Way Tallahassee, FL 32301 |
| 850.644.1829 |